Analysis by Flow Cytometry of α2-Macroglobulin and Nonimmune IgM-Binding to Plasmodium falciparum-Infected Erythrocytes.
Methods Mol Biol
; 2470: 435-444, 2022.
Article
em En
| MEDLINE
| ID: mdl-35881364
ABSTRACT
Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.
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Base de dados:
MEDLINE
Assunto principal:
Malária Falciparum
/
Alfa 2-Macroglobulinas Associadas à Gravidez
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article