Analysis of Antibody Inhibition of PfEMP1 Binding by Competition ELISA.
Methods Mol Biol
; 2470: 485-491, 2022.
Article
em En
| MEDLINE
| ID: mdl-35881368
ABSTRACT
Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.
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Base de dados:
MEDLINE
Assunto principal:
Malária Falciparum
/
Antígenos de Protozoários
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article