Recording gene expression order in DNA by CRISPR addition of retron barcodes.
Nature
; 608(7921): 217-225, 2022 08.
Article
em En
| MEDLINE
| ID: mdl-35896746
ABSTRACT
Biological processes depend on the differential expression of genes over time, but methods to make physical recordings of these processes are limited. Here we report a molecular system for making time-ordered recordings of transcriptional events into living genomes. We do this through engineered RNA barcodes, based on prokaryotic retrons1, that are reverse transcribed into DNA and integrated into the genome using the CRISPR-Cas system2. The unidirectional integration of barcodes by CRISPR integrases enables reconstruction of transcriptional event timing based on a physical record through simple, logical rules rather than relying on pretrained classifiers or post hoc inferential methods. For disambiguation in the field, we will refer to this system as a Retro-Cascorder.
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Base de dados:
MEDLINE
Assunto principal:
DNA
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RNA
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Expressão Gênica
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Armazenamento e Recuperação da Informação
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Transcrição Reversa
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Sistemas CRISPR-Cas
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Edição de Genes
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article