Dynamic in vitro hemocompatibility of oligoproline self-assembled monolayer surfaces.
Biomater Sci
; 10(19): 5498-5503, 2022 Sep 27.
Article
em En
| MEDLINE
| ID: mdl-35904349
ABSTRACT
The blood compatibility of self-assembled monolayers (SAMs) of oligoproline, a nonionic antifouling peptide, was investigated using the cone-and-plate assay imitating arterial blood flow conditions. End-capped oligoprolines composed of 6 and 9 proline residues (Pro6 and Pro9) and a Cys residue were synthesized for preparing SAMs (Pro-SAMs) on Au-sputtered glass. The surface of Pro-SAMs indicated hydrophilic property with a smooth topology. The adsorption of blood components and the adhesion of blood cells, including leukocytes and platelets, were strongly suppressed on Pro-SAMs. Moreover, Pro9-SAM did not trigger the activation of platelets (i.e., the conformational change of GPIIb/IIIa and P-selectin (CD62P) expression on platelets and the formation of aggregates). Our results demonstrate that Pro9-SAM completely inhibited acute thrombogenic responses and the activation of platelets under dynamic conditions.
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MEDLINE
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Plaquetas
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Selectina-P
Idioma:
En
Ano de publicação:
2022
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Article