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Bacterial biofilm functionalization through Bap amyloid engineering.
Matilla-Cuenca, Leticia; Taglialegna, Agustina; Gil, Carmen; Toledo-Arana, Alejandro; Lasa, Iñigo; Valle, Jaione.
Afiliação
  • Matilla-Cuenca L; Instituto de Agrobiotecnología (IDAB). CSIC- Gobierno de Navarra, Mutilva, Spain.
  • Taglialegna A; Instituto de Agrobiotecnología (IDAB). CSIC- Gobierno de Navarra, Mutilva, Spain.
  • Gil C; The Campus 4 Crinan Street London N1, London, UK.
  • Toledo-Arana A; Laboratory of Microbial Pathogenesis, Navarrabiomed, Hospital Universitario de Navarra (HUN), Universidad Pública de Navarra (UPNA), IdiSNA, Pamplona, Spain.
  • Lasa I; Instituto de Agrobiotecnología (IDAB). CSIC- Gobierno de Navarra, Mutilva, Spain.
  • Valle J; Laboratory of Microbial Pathogenesis, Navarrabiomed, Hospital Universitario de Navarra (HUN), Universidad Pública de Navarra (UPNA), IdiSNA, Pamplona, Spain.
NPJ Biofilms Microbiomes ; 8(1): 62, 2022 08 01.
Article em En | MEDLINE | ID: mdl-35909185
ABSTRACT
Biofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered to create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which play a dual role in biofilm formation by acting as adhesins in their native conformation and as matrix scaffolds when they polymerize into amyloid-like fibrillar structures, are interesting candidates. Here, we report the use of the facultative amyloid-like Bap protein of Staphylococcus aureus as a tool to decorate the extracellular biofilm matrix or the bacterial cell surface with a battery of functional domains or proteins. We demonstrate that the localization of the functional tags can be change by simply modulating the pH of the medium. Using Bap features, we build a tool for trapping and covalent immobilizing molecules at bacterial cell surface or at the biofilm matrix based on the SpyTag/SpyCatcher system. Finally, we show that the cell wall of several Gram-positive bacteria could be functionalized through the external addition of the recombinant engineered Bap-amyloid domain. Overall, this work shows a simple and modulable system for biofilm functionalization based on the facultative protein Bap.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Proteínas de Bactérias Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Proteínas de Bactérias Idioma: En Ano de publicação: 2022 Tipo de documento: Article