Production of long linear DNA substrates with site-specific chemical lesions for single-molecule replisome studies.

Kaur, Gurleen; Spenkelink, Lisanne M; Lewis, Jacob S; Jergic, Slobodan; Dixon, Nicholas E; van Oijen, Antoine M

Kaur, Gurleen; Spenkelink, Lisanne M; Lewis, Jacob S; Jergic, Slobodan; Dixon, Nicholas E; van Oijen, Antoine M.

Afiliação

Kaur G; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia; Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia.
Spenkelink LM; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia; Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia.
Lewis JS; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia; Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia.
Jergic S; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia; Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia.
Dixon NE; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia; Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia.
van Oijen AM; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia; Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia. Electronic address: vanoijen@uow.edu.au.

Methods Enzymol ; 672: 299-315, 2022.

Article em En | MEDLINE | ID: mdl-35934481

Assuntos

Replicação do DNA; DNA Polimerase Dirigida por DNA; DNA/metabolismo; DNA Polimerase III; DNA Polimerase Dirigida por DNA/química; Escherichia coli/genética; Escherichia coli/metabolismo

Palavras-chave

DNA purification methods; DNA replication; E.coli replisome; Single-molecule fluorescence imaging; Site-specific CPD lesions

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA Polimerase Dirigida por DNA / Replicação do DNA Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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