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Engineered multivalent DNA capsules for multiplexed detection of genotoxicants via versatile controlled release mechanisms.
Singuru, Murali Mohana Rao; Liao, Yu-Chieh; Lin, Gloria Meng-Hsuan; Chen, Wei-Tzu; Lin, Yu-Hsuan; To, Ching Tat; Liao, Wei-Ching; Hsu, Chun-Hua; Chuang, Min-Chieh.
Afiliação
  • Singuru MMR; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan.
  • Liao YC; Institute of Population Health Sciences, National Health Research Institutes, Miaoli, 35053, Taiwan.
  • Lin GM; Department of Agricultural Chemistry, National Taiwan University, Taipei, 10617, Taiwan.
  • Chen WT; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan.
  • Lin YH; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan.
  • To CT; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan.
  • Liao WC; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan.
  • Hsu CH; Department of Agricultural Chemistry, National Taiwan University, Taipei, 10617, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei, 10617, Taiwan.
  • Chuang MC; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan. Electronic address: mcchuang@thu.edu.tw.
Biosens Bioelectron ; 216: 114608, 2022 Nov 15.
Article em En | MEDLINE | ID: mdl-35961122
Assessing the risks associated with genotoxic compounds is challenging because of their complex genotoxicity and the difficulty in the dynamic monitoring of coexisting hazards. In this paper, DNA-assembly-based multistimulus responsive capsules that can detect multiple genotoxic agents simultaneously are presented. By exploiting the sequence- and reactivity-editable properties of DNA, DNA sequences in a DNA shell are designed to exhibit multivalent susceptibility against ultraviolet B radiation, aflatoxin B1, and styrene oxide. Upon exposure to genotoxicants, the developed DNA capsules dissociate because of the production of DNA adducts or aptamer-ligand complex-activated dehybridization, which results in the release of encapsulated fluorophores for a measure of the genotoxicant level. The fluorophore release kinetics for each genotoxicant is investigated. Moreover, the destruction behaviors of the developed capsules are evaluated in binary and ternary toxin mixtures. Multiple linear regression indicates the existence of a strong relationship between the fluorescent response and the genotoxicant level; the result highlights the significance of particular genotoxicant and the antagonistic effect of interacting genotoxic substances on capsule destruction. This DNA architecture allows the monitoring of human exposure to genotoxic agents, which enables the timely adoption of remedial measures, and benefits development of an endogenous genotoxin-responsive drug delivery system.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Aflatoxina B1 Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Aflatoxina B1 Idioma: En Ano de publicação: 2022 Tipo de documento: Article