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HLA molecular study of patients in a public kidney transplant program in Guatemala.
Escobar-Castro, Karla; Hernández-Zaragoza, Diana Iraiz; Santizo, Adolfo; Del Toro-Arreola, Susana; Hernández, Elisa; Toledo S, Manuel.
Afiliação
  • Escobar-Castro K; Escuela de Estudios de Postgrado, Facultad de Ciencias Médicas, Universidad de San Carlos de Guatemala (USAC), Guatemala City, Guatemala; Laboratorio de Histocompatibilidad, Departamento de Nefrología y Trasplante, Hospital General San Juan de Dios, Guatemala City, Guatemala.
  • Hernández-Zaragoza DI; Laboratorio de Genética Molecular, Escuela Nacional de Antropología e Historia (ENAH), Mexico City, Mexico; Unidad de Inmunogenética, Técnicas Genéticas Aplicadas a la Clínica (TGAC), Mexico City, Mexico. Electronic address: diiraiz3220@gmail.com.
  • Santizo A; Laboratorio de Histocompatibilidad, Departamento de Nefrología y Trasplante, Hospital General San Juan de Dios, Guatemala City, Guatemala.
  • Del Toro-Arreola S; Instituto de Investigación en Enfermedades Crónico-Degenerativas, Centro Universitario en Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
  • Hernández E; Laboratorio de Histocompatibilidad, Departamento de Nefrología y Trasplante, Hospital General San Juan de Dios, Guatemala City, Guatemala.
  • Toledo S M; Departamento de Nefrología y Trasplante, Hospital General San Juan de Dios, Guatemala City, Guatemala.
Hum Immunol ; 83(11): 741-748, 2022 Nov.
Article em En | MEDLINE | ID: mdl-36028459
Guatemala is a country located in Central America, and while it is one of the most populated countries in the region, the genetic diversity of the population has been poorly analyzed. Currently, there are no analyses of the distribution of human leukocyte antigen (HLA) system alleles in mixed ancestry (i.e., ladino) populations in Guatemala. The HLA system exhibits the most extensive polymorphism in the human genome and has been extensively analyzed in a large number of studies related to disease association, transplantation, and population genetics (with particular importance in the understanding of diversity in the human population). Here, we present HLA typing data from 127 samples of unrelated individuals from the kidney transplant program of the San Juan de Dios General Hospital (Guatemala City) using a PCR-SSOP-based (PCR-sequence specific oligonucleotide probes) typing method. We found 16 haplotypes that accounted for 39.76 % of the total haplotype diversity, of which thirteen have been reported previously in Native American populations and three have been reported in European populations. The analyses showed no deviations from Hardy-Weinberg equilibrium, and admixture estimates calculated with k = 3 ancestral components showed that Native American was the most represented component, followed by the European component. The African component was less prominent in the Guatemala mixed ancestry sample in comparison to samples from other countries in Central America. The HLA-based admixture results for Central America showed a continuum in the distribution of Native American, European and African ancestries throughout the region, which is consistent with the complex demographic history of the region.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transplante de Rim Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transplante de Rim Idioma: En Ano de publicação: 2022 Tipo de documento: Article