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Cadmium and copper mixture effects on immunological response and susceptibility to Vibrio harveyi in white shrimp Litopenaeus vannamei.
Bautista-Covarrubias, Juan Carlos; Valdez-Soto, Iriana Edith; Aguilar-Juárez, Marisela; Arreola-Hernández, Jonathan Omar; Soto-Jiménez, Martín Federico; Soto-Rodríguez, Sonia Araceli; López-Sánchez, José Armando; Osuna-Martínez, Carmen Cristina; Frías-Espericueta, Martín Gabriel.
Afiliação
  • Bautista-Covarrubias JC; Unidad Académica Escuela Nacional de Ingeniería Pesquera, Bahía de Matanchén. Universidad Autónoma de Nayarit. Tepic, Nayarit, C.P., 63740, Mexico.
  • Valdez-Soto IE; Facultad de Ciencias del Mar. Universidad Autónoma de Sinaloa, Mazatlán, Sinaloa, C.P., 82000, Mexico.
  • Aguilar-Juárez M; Facultad de Ciencias del Mar. Universidad Autónoma de Sinaloa, Mazatlán, Sinaloa, C.P., 82000, Mexico.
  • Arreola-Hernández JO; Facultad de Ciencias del Mar. Universidad Autónoma de Sinaloa, Mazatlán, Sinaloa, C.P., 82000, Mexico.
  • Soto-Jiménez MF; Unidad Académica Mazatlán, Instituto de Ciencias del Mar y Limnología, UNAM. Mazatlán Sinaloa, C.P., 82047, Mexico.
  • Soto-Rodríguez SA; Unidad Mazatlán, Centro de Investigación en Alimentación y Desarrollo, Mazatlán, Sinaloa, C.P., 82112, Mexico.
  • López-Sánchez JA; Unidad Académica Escuela Nacional de Ingeniería Pesquera, Bahía de Matanchén. Universidad Autónoma de Nayarit. Tepic, Nayarit, C.P., 63740, Mexico.
  • Osuna-Martínez CC; Facultad de Ciencias del Mar. Universidad Autónoma de Sinaloa, Mazatlán, Sinaloa, C.P., 82000, Mexico.
  • Frías-Espericueta MG; Facultad de Ciencias del Mar. Universidad Autónoma de Sinaloa, Mazatlán, Sinaloa, C.P., 82000, Mexico. Electronic address: friasm@uas.edu.mx.
Fish Shellfish Immunol ; 129: 145-151, 2022 Oct.
Article em En | MEDLINE | ID: mdl-36055556
ABSTRACT
Cadmium (Cd2+) and copper (Cu2+) are considered immunotoxic metals and their presence in combination in the aquatic environment may cause effects on shrimp species as Litopenaeus vannamei. Thus, this research evaluates the combined effects of Cd2+ and Cu2+ on shrimp inoculated with Vibrio harveyi bacteria. The experiments were performed at 96-h of exposure to sublethal concentrations of both metals. No mortality was observed in organisms exposed to the sum of Criterion of Continuous Concentration (ΣCCC) in Cd + Cu mixture and those inoculated with V. harveyi. Higher clotting times were recorded in Cd + Cu + V. harveyi treatment at higher metal concentrations. No significant differences (P > 0.05) were recorded in hemocyanin content between shrimp exposed to metals and those experimentally infected. Significantly higher (P < 0.05) total hemocyte count (THC) was recorded at 96 h exposure in the ΣCCC and 10% treatments of Cd + Cu + V. harveyi experiment. Regarding Cd + Cu + V. harveyi bioassay, the highest phenoloxidase (PO) activity was recorded in shrimp inoculated with V. harveyi (0.326 ± 0.031 PO units/mg protein) at 96-h exposure. The lowest PO activity was observed in organisms exposed to Cd + Cu + V. harveyi. Regarding superoxide dismutase (SOD) activity, shrimp exposed to higher metal concentrations at 96 h showed the lowest hemolymph activity (6.03 ± 0.62 SOD units/mL). Protein decrease was observed in organisms exposed to metal mixture. The results showed that L. vannamei could be more susceptible to V. harveyi when exposed to Cd + Cu.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cádmio / Penaeidae Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cádmio / Penaeidae Idioma: En Ano de publicação: 2022 Tipo de documento: Article