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Bacterial ectosymbionts in cuticular organs chemically protect a beetle during molting stages.
Janke, Rebekka S; Kaftan, Filip; Niehs, Sarah P; Scherlach, Kirstin; Rodrigues, Andre; Svatos, Ales; Hertweck, Christian; Kaltenpoth, Martin; Flórez, Laura V.
Afiliação
  • Janke RS; Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg University, 55128, Mainz, Germany.
  • Kaftan F; Department of Insect Symbiosis, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
  • Niehs SP; Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
  • Scherlach K; Department of Biomolecular Chemistry, Leibniz Institute for Natural Products Research and Infection Biology, HKI, 07745, Jena, Germany.
  • Rodrigues A; Department of Biomolecular Chemistry, Leibniz Institute for Natural Products Research and Infection Biology, HKI, 07745, Jena, Germany.
  • Svatos A; Department of Biochemistry and Microbiology, UNESP-São Paulo State University, Rio Claro, 13506-900 SP, Brazil.
  • Hertweck C; Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
  • Kaltenpoth M; Department of Biomolecular Chemistry, Leibniz Institute for Natural Products Research and Infection Biology, HKI, 07745, Jena, Germany.
  • Flórez LV; Institute of Microbiology, Faculty of Biological Sciences, Friedrich Schiller University Jena, 07745, Jena, Germany.
ISME J ; 16(12): 2691-2701, 2022 12.
Article em En | MEDLINE | ID: mdl-36056153
In invertebrates, the cuticle is the first and major protective barrier against predators and pathogen infections. While immune responses and behavioral defenses are also known to be important for insect protection, the potential of cuticle-associated microbial symbionts to aid in preventing pathogen entry during molting and throughout larval development remains unexplored. Here, we show that bacterial symbionts of the beetle Lagria villosa inhabit unusual dorsal invaginations of the insect cuticle, which remain open to the outer surface and persist throughout larval development. This specialized location enables the release of several symbiont cells and the associated protective compounds during molting. This facilitates ectosymbiont maintenance and extended defense during larval development against antagonistic fungi. One Burkholderia strain, which produces the antifungal compound lagriamide, dominates the community across all life stages, and removal of the community significantly impairs the survival probability of young larvae when exposed to different pathogenic fungi. We localize both the dominant bacterial strain and lagriamide on the surface of eggs, larvae, pupae, and on the inner surface of the molted cuticle (exuvia), supporting extended protection. These results highlight adaptations for effective defense of immature insects by cuticle-associated ectosymbionts, a potentially key advantage for a ground-dwelling insect when confronting pathogenic microbes.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Besouros / Burkholderia Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Besouros / Burkholderia Idioma: En Ano de publicação: 2022 Tipo de documento: Article