Event-driven acquisition for content-enriched microscopy.
Nat Methods
; 19(10): 1262-1267, 2022 10.
Article
em En
| MEDLINE
| ID: mdl-36076039
ABSTRACT
A common goal of fluorescence microscopy is to collect data on specific biological events. Yet, the event-specific content that can be collected from a sample is limited, especially for rare or stochastic processes. This is due in part to photobleaching and phototoxicity, which constrain imaging speed and duration. We developed an event-driven acquisition framework, in which neural-network-based recognition of specific biological events triggers real-time control in an instant structured illumination microscope. Our setup adapts acquisitions on-the-fly by switching between a slow imaging rate while detecting the onset of events, and a fast imaging rate during their progression. Thus, we capture mitochondrial and bacterial divisions at imaging rates that match their dynamic timescales, while extending overall imaging durations. Because event-driven acquisition allows the microscope to respond specifically to complex biological events, it acquires data enriched in relevant content.
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Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article