Reciprocal abrogation of PKM isoforms: contradictory outcomes and differing impact of splicing signal on CRISPR/Cas9 mediates gene editing in keratinocytes.

Nold, Simon P; Sych, Khrystyna; Imre, Gergely; Fuhrmann, Dominik C; Pfeilschifter, Josef; Vutukuri, Rajkumar; Schnutgen, Frank; Wittig, Ilka; Meisterknecht, Jana; Frank, Stefan; Goren, Itamar

Nold, Simon P; Sych, Khrystyna; Imre, Gergely; Fuhrmann, Dominik C; Pfeilschifter, Josef; Vutukuri, Rajkumar; Schnutgen, Frank; Wittig, Ilka; Meisterknecht, Jana; Frank, Stefan; Goren, Itamar.

Afiliação

Nold SP; Pharmazentrum Frankfurt/ZAFES, General Pharmacology and Toxicology, Faculty of Medicine, Goethe University Frankfurt, Germany.
Sych K; Pharmazentrum Frankfurt/ZAFES, General Pharmacology and Toxicology, Faculty of Medicine, Goethe University Frankfurt, Germany.
Imre G; Pharmazentrum Frankfurt/ZAFES, General Pharmacology and Toxicology, Faculty of Medicine, Goethe University Frankfurt, Germany.
Fuhrmann DC; Faculty of Medicine, Institute of Biochemistry I, Goethe University Frankfurt, Germany.
Pfeilschifter J; Pharmazentrum Frankfurt/ZAFES, General Pharmacology and Toxicology, Faculty of Medicine, Goethe University Frankfurt, Germany.
Vutukuri R; Pharmazentrum Frankfurt/ZAFES, General Pharmacology and Toxicology, Faculty of Medicine, Goethe University Frankfurt, Germany.
Schnutgen F; Hematology/Oncology, Faculty of Medicine 2, University Hospital, Goethe University Frankfurt, Germany.
Wittig I; German Cancer Research Center and German Cancer Consortium, Heidelberg, Germany.
Meisterknecht J; Frankfurt Cancer Institute (FCI), Germany.
Frank S; Functional Proteomics, Institute of Cardiovascular Physiology, Goethe University Frankfurt, Germany.
Goren I; German Centre for Cardiovascular Research (DZHK), Partner Site Rhein-Main, Frankfurt, Germany.

FEBS J ; 290(9): 2338-2365, 2023 05.

Article em En | MEDLINE | ID: mdl-36083715

Assuntos

Sistemas CRISPR-Cas; Edição de Genes; Sistemas CRISPR-Cas/genética; Isoformas de Proteínas/metabolismo; Splicing de RNA; Proteínas de Transporte/genética; Proteínas de Transporte/metabolismo; Piruvato Quinase/genética; Piruvato Quinase/metabolismo

Palavras-chave

HaCaT keratinocytes; genome editing; glutamine anaplerosis; minigene; splicing assay

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2023 Tipo de documento: Article