Mass spectrometry-based shotgun glycomics for discovery of natural ligands of glycan-binding proteins.
Curr Opin Struct Biol
; 77: 102448, 2022 Dec.
Article
em En
| MEDLINE
| ID: mdl-36088799
ABSTRACT
The non-covalent associations of complex carbohydrates (glycans) with glycan-binding proteins mediate many important physiological and pathophysiological processes. Identifying these interactions is essential to understanding their diverse biological functions and enables the development of new disease treatments and diagnostics. Knowledge of the repertoire of glycans recognized by most glycan-binding proteins and their affinities is incomplete. Mass spectrometry-based screening of natural glycan libraries has emerged as a promising approach to defining the glycan interactome of glycan-binding proteins. Here, we review recent advances in mass spectrometry-based natural library screening that have led to the discovery of glycan ligands of endogenous and exogenous proteins and illuminated their binding specificities.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Glicômica
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article