Sequential in vitro enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing.

Adams, Trevor M; Zhao, Peng; Chapla, Digantkumar; Moremen, Kelley W; Wells, Lance

Sequential in vitro enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing.

Adams, Trevor M; Zhao, Peng; Chapla, Digantkumar; Moremen, Kelley W; Wells, Lance.

Afiliação

Adams TM; Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
Zhao P; Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
Chapla D; Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
Moremen KW; Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA. Electronic address: moremen@uga.edu.
Wells L; Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA. Electronic address: lwells@ccrc.uga.edu.

J Biol Chem ; 298(10): 102474, 2022 10.

Article em En | MEDLINE | ID: mdl-36089065

Assuntos

Glicoproteínas; Espectrometria de Massas em Tandem; Humanos; Cromatografia Líquida; Glicoproteínas/química; Glicoproteínas/metabolismo; Células HEK293; Polissacarídeos/química; Polissacarídeos/metabolismo; Glicosilação

Palavras-chave

glycomics; glycoprotein biosynthesis; glycosylation; glycosyltransferase; microheterogeneity; substrate specificity

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Glicoproteínas / Espectrometria de Massas em Tandem Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google