Maternal pre-pregnancy body mass index and offspring with overweight/obesity at preschool age: The possible role of epigenome-wide DNA methylation changes in cord blood.
Pediatr Obes
; 18(1): e12969, 2023 01.
Article
em En
| MEDLINE
| ID: mdl-36102013
ABSTRACT
BACKGROUND:
Epigenome-wide association studies have identified some DNA methylation sites associated with body mass index (BMI) or obesity. Studies in the Asian population are lacking.OBJECTIVE:
To examine the association of cord blood genome-wide DNA methylation (GWDm) changes with maternal pre-pregnancy BMI and children's BMI-z score at preschool age. Additionally, we also explored the genome-wide differentially methylated regions and differentially methylated probes between preschoolers with overweight/obesity and normal-weight counterparts.METHODS:
This two-stage study design included (1) a GWDm analysis of 30 mother-child pairs from 633 participants of the Zhuhai birth cohort with data on newborn cord blood, maternal pre-pregnancy BMI, and children's BMI at 3 years of age; and (2) a targeted validation analysis of the cord blood of ten children with overweight/obesity and ten matched controls to validate the CpG sites.RESULTS:
In the first stage, no significant CpG sites were found to be associated with children's BMI-z score at preschool age after FDR correction with the p-values of the CpG sites in FOXN3 (cg23501836) and ZNF264 (cg27437574) being close to 1 × 10-6 . In the second stage, a significant difference of CpG sites in AHRR (chr5355067-355068) and FOXN3 (chr14 89630264-89630272 and chr14 89630387-89630388) was found between the ten children with overweight/obesity and ten controls (p < 0.05). The CpG sites in FOXN3 (chr1489630264-89630272 and chr1489630295-89630296) and ZNF264 (chr19 57703104-57703107 and chr19 57703301-57703307) were associated with children's BMI-z score; and the CpG sites in FOXN3 (chr14 89630264-89630272 and chr14 89630387-89630388) were associated with maternal pre-pregnancy BMI.CONCLUSIONS:
DNA methylation in FOXN3 and AHRR is associated with overweight/obesity in preschool-aged children, and the methylation in FOXN3 and ZNF264 might be associated with children's BMI-z score. FOXN3 methylation may be associated with maternal pre-pregnancy BMI, suggesting its potential role in the children's BMI-z score or overweight/obesity. Our results provide novel insights into the mechanisms of children's obesity.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Metilação de DNA
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Sobrepeso
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article