MicroRNAs combined with the TLR4/TDAG8 mRNAs and proinflammatory cytokines are biomarkers for the rapid diagnosis of sepsis.
Mol Med Rep
; 26(5)2022 11.
Article
em En
| MEDLINE
| ID: mdl-36102304
ABSTRACT
The early diagnosis and treatment of sepsis are of particular importance to patient survival. To obtain novel biomarkers that serve as prompt indicators of sepsis, the current study screened the differentially expressed microRNAs (DEMs) that were associated with sepsis susceptibility. The correlation between the elucidated DEMs and the inflammatory response was also examined. The present study included 40 patients with sepsis and 40 healthy controls. RNAsequencing technology and bioinformatics analysis were applied to screen the DEMs between the two cohorts. The expression of these DEMs was subsequently verified by performing reverse transcriptionquantitative PCR (RTqPCR). In addition, IL6, IL21, CXC motif chemokine ligand8 (CXCL8) and monocyte chemoattractant protein1 (MCP1) levels, along with Tcell deathassociated gene 8 (TDAG8) and tolllike receptor 4 (TLR4) mRNA expression levels were assessed. The association between microRNA (miRNA/miR)36633p and the secretion of various proinflammatory cytokines or TDAG8 and TLR4 mRNA expressions were subsequently evaluated by linear correlation analysis. The results revealed 305 DEMs (P<0.05; fold change >2) between patients with sepsis and healthy controls. Among these, the top 18 up and downregulated miRNAs were selected for RTqPCR verification. In addition, the serum content of IL6, IL21, CXCL8 and MCP1, and the expression of TDAG8 and TLR4 mRNAs were significantly increased in patients with sepsis compared with healthy controls. Moreover, in patients with sepsis, a positive correlation was identified between miR36633p and the secretion of inflammatory cytokines or TDAG8 and TLR4 mRNA expression. A positive correlation was also elucidated between TDAG8 and TLR4 mRNA expression and proinflammatory cytokine/chemokine secretion. Receiver operating characteristic curve analysis of miR36633p expression, IL6, IL21, CXCL8 and MCP1 secretion and TDAG8 and TLR4 mRNA expression demonstrated that miRNA analysis may be invaluable for the diagnosis of sepsis. Collectively, the results determined that miR36633p may be a potentially powerful diagnostic and predictive biomarker of sepsis and that the combined and simultaneous detection of several biomarkers, including proteins, miRNAs and mRNA may be a reliable approach for the fast diagnosis and early identification of sepsis.
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MEDLINE
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Sepse
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MicroRNAs
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Receptores Acoplados a Proteínas G
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article