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Exploring the Healthy Eye Microbiota Niche in a Multicenter Study.
Borroni, Davide; Paytuví-Gallart, Andreu; Sanseverino, Walter; Gómez-Huertas, Carmen; Bonci, Paola; Romano, Vito; Giannaccare, Giuseppe; Rechichi, Miguel; Meduri, Alessandro; Oliverio, Giovanni William; Rocha-de-Lossada, Carlos.
Afiliação
  • Borroni D; Department of Doctoral Studies, Riga Stradins University, LV-1007 Riga, Latvia.
  • Paytuví-Gallart A; Eyemetagenomics Ltd., 71-75, Shelton Street, Covent Garden, London WC2H 9JQ, UK.
  • Sanseverino W; Sequentia Biotech SL, Carrer del Dr. Trueta, 179, 08005 Barcelona, Spain.
  • Gómez-Huertas C; Sequentia Biotech SL, Carrer del Dr. Trueta, 179, 08005 Barcelona, Spain.
  • Bonci P; Department of Ophthalmology, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain.
  • Romano V; Ospedale Civile di Ravenna, Banca Delle Cornee Della Regione Emilia-Romagna, 48121 Ravenna, Italy.
  • Giannaccare G; Department of Medical and Surgical Specialties, Radiological Specialties and Public Health, 9297 University of Brescia, ASST Spedali Civili, 25100 Brescia, Italy.
  • Rechichi M; Department of Ophthalmology, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italy.
  • Meduri A; Centro Polispecialistico Mediterraneo, 88050 Sellia Marina, Italy.
  • Oliverio GW; Biomedical Science Department, Institute of Ophthalmology, University of Messina, Via Consolare Valeria, 98146 Messina, Italy.
  • Rocha-de-Lossada C; Biomedical Science Department, Institute of Ophthalmology, University of Messina, Via Consolare Valeria, 98146 Messina, Italy.
  • On Behalf Of Lucy Consortium; Eyemetagenomics Ltd., 71-75, Shelton Street, Covent Garden, London WC2H 9JQ, UK.
Int J Mol Sci ; 23(18)2022 Sep 06.
Article em En | MEDLINE | ID: mdl-36142138
ABSTRACT

PURPOSE:

This study aims to explore and characterize healthy eye microbiota.

METHODS:

Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end-repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02).

RESULTS:

Healthy eye microbiota is a low-diversity microbiome. The vast majority of the 137 analyzed samples were highly enriched with Staphylococcus, whereas only in a few of them, other genera such as Bacillus, Pseudomonas, and Corynebacterium predominate. We found an average of 88 genera with an average Shannon index of 0.65.

CONCLUSION:

We identified nine different ECSTs. A better understanding of healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to promote health.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbiota / Promoção da Saúde Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbiota / Promoção da Saúde Idioma: En Ano de publicação: 2022 Tipo de documento: Article