Uranyl-Free Staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy.
Methods Mol Biol
; 2566: 225-231, 2023.
Article
em En
| MEDLINE
| ID: mdl-36152255
ABSTRACT
Uranyl acetate solution has widely been used as staining reagent for samples processed for ultrastructural morphology, cytochemistry, and immunocytochemistry. Although uranyl acetate guarantees high performance as a staining reagent, the radioactive uranyl salts make its use and purchase severely restricted. In this view, we used a non-radioactive lanthanide mix solution as contrasting dye for both nucleoplasmic and nucleolar ribonucleoprotein-containing components. This method guarantees a good contrast without masking the probe-antigen immunoreaction, thus proving to be a suitable tool for high-resolution studies of both cyto- and immunocytochemistry on acrylic resin-embedded samples.
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MEDLINE
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Sais
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Elementos da Série dos Lantanídeos
Idioma:
En
Ano de publicação:
2023
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Article