Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil.
Rev Soc Bras Med Trop
; 55: e0067, 2022.
Article
em En
| MEDLINE
| ID: mdl-36169488
ABSTRACT
BACKGROUND:
Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear.METHODS:
We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing.RESULTS:
Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV).CONCLUSIONS:
The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Flaviviridae
/
Culex
/
Flavivirus
/
Culicidae
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article