Solid-Phase Binding Assay for Ganglioside Binding of Human Respiroviruses.
Methods Mol Biol
; 2556: 179-186, 2022.
Article
em En
| MEDLINE
| ID: mdl-36175635
ABSTRACT
Human parainfluenza virus types 1 (hPIV-1) and 3 (hPIV-3) belong to the family Paramyxoviridae, subfamily Paramyxoviridae, and genus Respirovirus. The viruses enter by utilizing glycoproteins or glycosphingolipids (gangliosides) containing sialic acid on the cell membrane. We developed a solid-phase binding assay to evaluate hPIV-1, hPIV-3, and Sendai virus' abilities to bind to different types of gangliosides. hPIV1 and hPIV3 show strong binding to neolacto-series gangliosides containing a non-reducing terminal sialic acid residue and different specificity regarding the sialic acid linkages. This solid-phase binding assay is suitable to evaluate other orthomyxoviruses and paramyxoviruses' binding specificities utilizing sialic acids.
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1
Base de dados:
MEDLINE
Assunto principal:
Respirovirus
/
Ácido N-Acetilneuramínico
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article