SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules.
Proc Natl Acad Sci U S A
; 119(41): e2207605119, 2022 10 11.
Article
em En
| MEDLINE
| ID: mdl-36191189
ABSTRACT
The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients.
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MEDLINE
Assunto principal:
Sêmen
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Ciliopatias
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article