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Evolution of increased complexity and specificity at the dawn of form I Rubiscos.
Schulz, Luca; Guo, Zhijun; Zarzycki, Jan; Steinchen, Wieland; Schuller, Jan M; Heimerl, Thomas; Prinz, Simone; Mueller-Cajar, Oliver; Erb, Tobias J; Hochberg, Georg K A.
Afiliação
  • Schulz L; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.
  • Guo Z; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
  • Zarzycki J; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.
  • Steinchen W; Center for Synthetic Microbiology (SYNMIKRO), Philipps University Marburg, 35043 Marburg, Germany.
  • Schuller JM; Department of Chemistry, Philipps University Marburg, 35043 Marburg, Germany.
  • Heimerl T; Center for Synthetic Microbiology (SYNMIKRO), Philipps University Marburg, 35043 Marburg, Germany.
  • Prinz S; Department of Chemistry, Philipps University Marburg, 35043 Marburg, Germany.
  • Mueller-Cajar O; Center for Synthetic Microbiology (SYNMIKRO), Philipps University Marburg, 35043 Marburg, Germany.
  • Erb TJ; Department of Biology, Philipps University Marburg, 35043 Marburg, Germany.
  • Hochberg GKA; Central Electron Microscopy Facility, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany.
Science ; 378(6616): 155-160, 2022 10 14.
Article em En | MEDLINE | ID: mdl-36227987
The evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an important event for photosynthetic organisms adapting to an oxygenated environment. We use ancestral sequence reconstruction to recapitulate this event. We show that Rubisco increased its specificity and carboxylation efficiency through the gain of an accessory subunit before atmospheric oxygen was present. Using structural and biochemical approaches, we retrace how this subunit was gained and became essential. Our work illuminates the emergence of an adaptation to rising ambient oxygen levels, provides a template for investigating the function of interactions that have remained elusive because of their essentiality, and sheds light on the determinants of specificity in Rubisco.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ribulose-Bifosfato Carboxilase / Dióxido de Carbono / Evolução Molecular / Domínio Catalítico Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ribulose-Bifosfato Carboxilase / Dióxido de Carbono / Evolução Molecular / Domínio Catalítico Idioma: En Ano de publicação: 2022 Tipo de documento: Article