Endothelial-mesenchymal transition as a novel mechanism for generating myofibroblasts during wound healing and scarring.
J Cosmet Dermatol
; 22(2): 661-668, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36237150
ABSTRACT
BACKGROUND:
The endothelial-mesenchymal transition (EndMT) is an important mechanism in tissue regeneration and the development of organ fibrosis. Whether EndMT occurs in wound healing and scarring remains unknown. MATERIALS ANDMETHODS:
The isolated cells from the normal dermal tissue and the wound tissue of mouse with full-thickness skin wound, and human scar tissue sections were performed with CD31/factorVII and α-SMA immunohistochemical staining and H and E staining. The ratio of factor VII or CD31/α-SMA double-positive cells in factor VII-positive cells was assessed in the isolated cells and in scar tissues.RESULTS:
In this study, we found that approximately 27-60% of ECs coexpressed VII factor and α-SMA in the isolated cells from the wound tissues of mice, which was significantly higher than that of normal dermal tissue cells. Accordingly, the number of CD31/α-SMA double-positive cells in mouse wound tissue sections was also significantly more than that in normal dermal tissue sections. In scar tissues, in addition to high-density microvessels, a large number of proliferative ECs in scar strama and CD31/α-SMA double-positive cells were also found. Approximately 46.82 to 84.11% of ECs and 68.77 to 95.25% of myofibroblasts coexpressed VII factor and α-SMA, and these two values in hypertrophic scars were significantly higher than those in keloids.CONCLUSION:
These results confirmed that ECs might contribute to the emergence of myofibroblasts in the wound and scar tissue via the process of EndMT.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Cicatriz Hipertrófica
/
Queloide
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article