Applying a Knock-In Strategy to Create Reporter-Tagged Knockout Alleles in Axolotls.

Wang, Liqun; Zeng, Yan-Yun; Liu, Yanmei; Fei, Ji-Feng

Wang, Liqun; Zeng, Yan-Yun; Liu, Yanmei; Fei, Ji-Feng.

Afiliação

Wang L; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou, China.
Zeng YY; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou, China.
Liu Y; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou, China. yanmeiliu@m.scnu.edu.cn.
Fei JF; Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China. jifengfei@gdph.org.cn.

Methods Mol Biol ; 2562: 351-368, 2023.

Article em En | MEDLINE | ID: mdl-36272087

Assuntos

Ambystoma mexicanum; Sistemas CRISPR-Cas; Animais; Ambystoma mexicanum/genética; Sistemas CRISPR-Cas/genética; Alelos; Técnicas de Introdução de Genes; Técnicas de Inativação de Genes

Palavras-chave

Axolotl; CRISPR/Cas9; Genomic editing; Knock-in; Knockout; Pax7

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Ambystoma mexicanum Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Ambystoma mexicanum Idioma: En Ano de publicação: 2023 Tipo de documento: Article