Cholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR.
Biosensors (Basel)
; 12(10)2022 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-36290926
ABSTRACT
Cholesterol, an important lipid in animal membranes, binds to hydrophobic pockets within many soluble proteins, transport proteins and membrane bound proteins. The study of cholesterol-protein interactions in aqueous solutions is complicated by cholesterol's low solubility and often requires organic co-solvents or surfactant additives. We report the synthesis of a biotinylated cholesterol and immobilization of this derivative on a streptavidin chip. Surface plasmon resonance (SPR) was then used to measure the kinetics of cholesterol interaction with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B.
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Base de dados:
MEDLINE
Assunto principal:
Ressonância de Plasmônio de Superfície
/
Proteínas Hedgehog
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article