Improving the growth of Spirulina in CO2 absorption and microalgae conversion (CAMC) system through mixotrophic cultivation: Reveal of metabolomics.

Mixotrophic cultivation was proposed to enhance the biomass and carbon sequestration efficiency of Spirulina in CO2 absorption and microalgae conversion (CAMC) system, and the underlying metabolic mechanism was also explored. The result showed that mixotrophic enhanced the performance of CAMC system, the maximum biomass, total carbon conversion capacity and efficiency was obtained at 0.5 g/L acetate group, which was 60.47 %, 63.06 % and 59.77 % higher than control. Adding 0.5 g/L acetate enhanced the activities of Rubisco and Acetyl-CoA, arrived at 89.59 U/g and 5.16 nmol/g, respectively. Metabolomics analyses suggested that mixotrophic changed metabolic flux and affected intracellular composition. Mixotrophic up-regulated Calvin cycle, glycolysis, and tricarboxylic acid (TCA) cycle, induced more carbon fluxes into central carbon metabolism for the growth of Spirulina. These results suggested that mixotrophic could supply effective energy and carbon skeleton for rapid growth of Spirulina, and provided a theoretical basis for large-scale application of CAMC system.