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The Stress Response of the Holothurian Central Nervous System: A Transcriptomic Analysis.
Cruz-González, Sebastián; Quesada-Díaz, Eduardo; Miranda-Negrón, Yamil; García-Rosario, Raúl; Ortiz-Zuazaga, Humberto; García-Arrarás, José E.
Afiliação
  • Cruz-González S; Department of Biology, College of Natural Sciences, University of Puerto Rico, Río Piedras, San Juan, PR 00925, USA.
  • Quesada-Díaz E; Department of Biology, College of Natural Sciences, University of Puerto Rico, Río Piedras, San Juan, PR 00925, USA.
  • Miranda-Negrón Y; Department of Biology, College of Natural Sciences, University of Puerto Rico, Río Piedras, San Juan, PR 00925, USA.
  • García-Rosario R; Department of Biology, College of Natural Sciences, University of Puerto Rico, Río Piedras, San Juan, PR 00925, USA.
  • Ortiz-Zuazaga H; Department of Computer Science, College of Natural Sciences, University of Puerto Rico, Río Piedras, San Juan, PR 00925, USA.
  • García-Arrarás JE; Department of Biology, College of Natural Sciences, University of Puerto Rico, Río Piedras, San Juan, PR 00925, USA.
Int J Mol Sci ; 23(21)2022 Nov 02.
Article em En | MEDLINE | ID: mdl-36362181
ABSTRACT
Injury to the central nervous system (CNS) results in permanent damage and lack of function in most vertebrate animals, due to their limited regenerative capacities. In contrast, echinoderms can fully regenerate their radial nerve cord (RNC) following transection, with little to no scarring. Investigators have associated the regenerative capacity of some organisms to the stress response and inflammation produced by the injury. Here, we explore the gene activation profile of the stressed holothurian CNS. To do this, we performed RNA sequencing on isolated RNC explants submitted to the stress of transection and enzyme dissection and compared them with explants kept in culture for 3 days following dissection. We describe stress-associated genes, including members of heat-shock families, ubiquitin-related pathways, transposons, and apoptosis that were differentially expressed. Surprisingly, the stress response does not induce apoptosis in this system. Other genes associated with stress in other animal models, such as hero proteins and those associated with the integrated stress response, were not found to be differentially expressed either. Our results provide a new viewpoint on the stress response in the nervous system of an organism with amazing regenerative capacities. This is the first step in deciphering the molecular processes that allow echinoderms to undergo fully functional CNS regeneration, and also provides a comparative view of the stress response in other organisms.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Transcriptoma Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Transcriptoma Idioma: En Ano de publicação: 2022 Tipo de documento: Article