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In Vitro (Trans)Migration Experiment Using Chemokines as Stimulatory Factor.
Maas, Sanne L; van der Vorst, Emiel P C.
Afiliação
  • Maas SL; Interdisciplinary Centre for Clinical Research (IZKF), RWTH Aachen University, Aachen, Germany.
  • van der Vorst EPC; Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, Aachen, Germany.
Methods Mol Biol ; 2597: 77-87, 2023.
Article em En | MEDLINE | ID: mdl-36374415
ABSTRACT
Transmigration assays, and the use of the Boyden chamber, became one of the most used tools to assess cell motility, invasion, and chemotaxis. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier, which cells have to overcome by active migration. A large variety of Boyden chambers are available and can be customized to fit the experiment by choosing pore size, density, and membrane type. The method described in this chapter intends to measure the migration of mouse T cells towards the chemoattractant CCL25, as a practical example of such (trans)migration experiment that can be further adopted to individual needs and requirements.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Quimiocinas Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Quimiocinas Idioma: En Ano de publicação: 2023 Tipo de documento: Article