Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion.
Chembiochem
; 24(3): e202200463, 2023 02 01.
Article
em En
| MEDLINE
| ID: mdl-36420784
ABSTRACT
The highly glycosylated spike protein of SARS-CoV-2 is essential for infection and constitutes a prime target for antiviral agents and vaccines. The pineapple-derived jacalin-related lectin AcmJRL is present in the medication bromelain in significant quantities and has previously been described to bind mannosides. Here, we performed a large ligand screening of AcmJRL by glycan array analysis, quantified the interaction with carbohydrates and validated high-mannose glycans as preferred ligands. Because the SARS-CoV-2 spike protein was previously reported to carry a high proportion of high-mannose N-glycans, we tested the binding of AcmJRL to the recombinantly produced extraviral domain of spike protein. We could demonstrate that AcmJRL binds the spike protein with a low-micromolar KD in a carbohydrate-dependent fashion.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ananas
/
Glicoproteína da Espícula de Coronavírus
/
SARS-CoV-2
/
Lectinas
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article