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Splice variants of protein disulfide isomerase - identification, distribution and functional characterization in the rat.
Chetot, Thomas; Serfaty, Xavier; Carret, Léna; Kriznik, Alexandre; Grand, Lucie; Jacolot, Maïwenn; Popowycz, Florence; Benoit, Etienne; Lambert, Véronique; Lattard, Virginie.
Afiliação
  • Chetot T; USC 1233 RS2GP, VetAgro Sup, INRAe, Université de Lyon, 69280 Marcy l'étoile, France.
  • Serfaty X; USC 1233 RS2GP, VetAgro Sup, INRAe, Université de Lyon, 69280 Marcy l'étoile, France.
  • Carret L; USC 1233 RS2GP, VetAgro Sup, INRAe, Université de Lyon, 69280 Marcy l'étoile, France.
  • Kriznik A; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France.
  • Sophie-Rahuel-Clermont; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France.
  • Grand L; Univ Lyon, INSA Lyon, Université Lyon 1, CNRS, CPE Lyon, UMR 5246, ICBMS, 69621 Villeurbanne Cedex, France.
  • Jacolot M; Univ Lyon, INSA Lyon, Université Lyon 1, CNRS, CPE Lyon, UMR 5246, ICBMS, 69621 Villeurbanne Cedex, France.
  • Popowycz F; Univ Lyon, INSA Lyon, Université Lyon 1, CNRS, CPE Lyon, UMR 5246, ICBMS, 69621 Villeurbanne Cedex, France.
  • Benoit E; USC 1233 RS2GP, VetAgro Sup, INRAe, Université de Lyon, 69280 Marcy l'étoile, France.
  • Lambert V; USC 1233 RS2GP, VetAgro Sup, INRAe, Université de Lyon, 69280 Marcy l'étoile, France.
  • Lattard V; USC 1233 RS2GP, VetAgro Sup, INRAe, Université de Lyon, 69280 Marcy l'étoile, France. Electronic address: virginie.lattard@vetagro-sup.fr.
Biochim Biophys Acta Gen Subj ; 1867(2): 130280, 2023 02.
Article em En | MEDLINE | ID: mdl-36423740
BACKGROUND: Protein Disulfide Isomerase (PDI) enzyme is an emerging therapeutic target in oncology and hematology. Although PDI reductase activity has been studied with isolated fragments of the protein, natural structural variations affecting reductase activity have not been addressed. METHODS: In this study, we discovered four coding splice variants of the Pdi pre-mRNA in rats. In vitro Michaelis constants and apparent maximum steady-state rate constants after purification and distribution in different rat tissues were determined. RESULTS: The consensus sequence was found to be the most expressed splice variant while the second most expressed variant represents 15 to 35% of total Pdi mRNA. The third variant shows a quasi-null expression profile and the fourth was not quantifiable. The consensus sequence splice variant and the second splice variant are widely expressed (transcription level) in the liver and even more present in males. Measurements of the reductase activity of recombinant PDI indicate that the consensus sequence and third splice variant are fully active variants. The second most expressed variant, differing by a lack of signal peptide, was found active but less than the consensus sequence. GENERAL SIGNIFICANCE: Our work emphasizes the importance of taking splice variants into account when studying PDI-like proteins to understand the full biological functionalities of PDI.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sinais Direcionadores de Proteínas / Isomerases de Dissulfetos de Proteínas Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sinais Direcionadores de Proteínas / Isomerases de Dissulfetos de Proteínas Idioma: En Ano de publicação: 2023 Tipo de documento: Article