Expansion Microscopy of Bacillus subtilis.
Methods Mol Biol
; 2601: 191-202, 2023.
Article
em En
| MEDLINE
| ID: mdl-36445585
ABSTRACT
Expansion microscopy enables super-resolved visualization of specimen without the need of highly sophisticated and expensive optical instruments. Instead, the method is executed with conventional chemicals and lab equipment. Imaging of bacteria is performed using standard fluorescence microscopy. This chapter describes a protocol for the expansion microscopy of Bacillus subtilis expressing DivIVA-GFP. In addition, the cell wall was labeled by wheat germ agglutinin. Here, we place emphasis on the challenges of selecting the protein and organism of interest.
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Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
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Parede Celular
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article