Structural Identification of Eicosanoids with Ring Structures Using Differential Mobility Spectrometry-Electron Impact Excitation of Ions from Organics Mass Spectrometry.
J Am Soc Mass Spectrom
; 34(1): 75-81, 2023 Jan 04.
Article
em En
| MEDLINE
| ID: mdl-36507839
ABSTRACT
We developed a structural identification method for eicosanoids with various ring structures using mass spectrometry. We discovered that an electron beam with a kinetic energy of 10 eV, which is in the Electron Impact Excitation of Ions from Organics (EIEIO) regime, cleaved the fatty acids enough to distinguish constitutional and cis/trans isomers. In addition to EIEIO, a comparison to authentic standards using differential mobility spectrometry (DMS) can identify diastereomers, which was difficult by EIEIO. The combination of EIEIO and DMS can provide a high-throughput method to identify complete structures of eicosanoids in mixed samples, which is not allowed with conventional analytical methods though eicosanoids are important signaling molecules in biosystems.
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1
Base de dados:
MEDLINE
Assunto principal:
Eicosanoides
/
Elétrons
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article