Electrochemical Single-Cell Protein Therapeutics Using a Double-Barrel Nanopipette.
Angew Chem Int Ed Engl
; 62(9): e202215801, 2023 02 20.
Article
em En
| MEDLINE
| ID: mdl-36550087
ABSTRACT
Single-cell protein therapeutics is expected to promote our in-depth understanding of how a specific protein with a therapeutic dosage treats the cell without population averaging. However, it has not yet been tackled by current single-cell nanotools. We address this challenge by the use of a double-barrel nanopipette, in which one lumen was used for electroosmotic cytosolic protein delivery and the other was customized for ionic evaluation of the consequence. Upon injection of protein DJ-1 through the delivery lumen, upregulation of the antioxidant protein could protect neural PC-12 cells against oxidative stress from phorbol myristate acetate exposure, as deduced by targeting of the cytosolic hydrogen peroxide by the detecting lumen. The nanotool developed in this study for single-cell protein therapeutics provides a perspective for future single-cell therapeutics involving different therapeutic modalities, such as peptides, enzymes and nucleic acids.
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Base de dados:
MEDLINE
Assunto principal:
Terapia Baseada em Transplante de Células e Tecidos
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Proteína Desglicase DJ-1
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article