Atypical Mutational Spectrum of SARS-CoV-2 Replicating in the Presence of Ribavirin.
Antimicrob Agents Chemother
; 67(1): e0131522, 2023 01 24.
Article
em En
| MEDLINE
| ID: mdl-36602354
ABSTRACT
We report that ribavirin exerts an inhibitory and mutagenic activity on SARS-CoV-2-infecting Vero cells, with a therapeutic index higher than 10. Deep sequencing analysis of the mutant spectrum of SARS-CoV-2 replicating in the absence or presence of ribavirin indicated an increase in the number of mutations, but not in deletions, and modification of diversity indices, expected from a mutagenic activity. Notably, the major mutation types enhanced by replication in the presence of ribavirin were AâG and UâC transitions, a pattern which is opposite to the dominance of GâA and CâU transitions previously described for most RNA viruses. Implications of the inhibitory activity of ribavirin, and the atypical mutational bias produced on SARS-CoV-2, for the search for synergistic anti-COVID-19 lethal mutagen combinations are discussed.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribavirina
/
COVID-19
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article