Expansion microscopy of the chick embryo neural tube to overcome molecular crowding at the centrosomes-cilia.
STAR Protoc
; 4(1): 101997, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36609151
ABSTRACT
We describe an optimized protocol for application of expansion microscopy (ExM) on chick neural tube (NT) which enables different oriented nanoscale resolution imaging of the centrosomes/cilia. We explain embryo NT transversal sections and open-book preparations, immunohistochemistry for labeling, and sample preparation for 5-fold tissue expansion. Further, we detail sample orientation and Fast Airyscan confocal acquisition and show that NT-ExM retains fluorescence signals and overcomes biomolecules crowding in structural features that to date were only imaged with electron microscopy on tissues.
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Microscopia
Idioma:
En
Ano de publicação:
2023
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Article