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A novel exposure mode based on UVA-LEDs for bacterial inactivation.
Zhao, Ning; Lv, Li-Ping; Ma, Ping; Zhang, Yang-Yang; Deng, Jiang; Zhang, Yan-Yu.
Afiliação
  • Zhao N; Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.; Key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, People's Republic of China.
  • Lv LP; Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.; Key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, People's Republic of China.
  • Ma P; Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.; Key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, People's Republic of China.
  • Zhang YY; Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.; Key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, People's Republic of China.
  • Deng J; Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.; Key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, People's Republic of China.. Electronic address: ammsdjxm@163.com.
  • Zhang YY; Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China.; Key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, People's Republic of China.. Electronic address: swgczhyy@126.com.
J Photochem Photobiol B ; 239: 112641, 2023 Feb.
Article em En | MEDLINE | ID: mdl-36610349
As an emerging UV source, ultraviolet light-emitting diodes (UV-LEDs) are increasingly being used for disinfection purposes. UVA-LEDs have a higher output power, lower cost, and stronger penetration and cause less harm than UVC-LEDs. In this study, a novel exposure mode based on UVA was proposed and well demonstrated by various experiments using S. aureus as an indicator. Compared with single-dose exposure, fractionated exposure with a 15 min interval between treatments resulted in increased S. aureus inactivation. A longer interval or lower first irradiation dose was unfavorable for inactivation. Fractionated exposure changed the inactivation rate constant and eliminated the shoulder in the fluence-response curves. This resulted in changing the sensitivity of bacteria to UVA and improving bacterial inactivation. Moreover, the fractioned exposure mode has universality for various bacteria (including gram-positive and gram-negative bacteria). S. aureus was not reactivated by photoreactivation or dark repair after UVA treatment. As expected, the cells were damaged more seriously after fractionated exposure, further suggesting the advantages of this new exposure mode. In addition, the mechanism by which bacteria were inactivated after fractionated exposure was investigated, and it was found that •OH played an important role. A longer interval between treatments showed an adverse effect on inactivation, mainly due to the reduction of •OH and recovery of intracellular GSH. In summary, the current work provides novel ideas for the application of UVA-LEDs, which will give more choices for disinfection treatment.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias Gram-Negativas / Antibacterianos Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias Gram-Negativas / Antibacterianos Idioma: En Ano de publicação: 2023 Tipo de documento: Article