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UHPLC-Triple-TOF-MS Characterization, Antioxidant, Antimicrobial and Antiproliferative Activity of Raspberry (Rubus idaeus L.) Seed Extracts.
Maric, Bosko; Abramovic, Biljana; Ilic, Nebojsa; Bodroza-Solarov, Marija; Pavlic, Branimir; Oczkowski, Michal; Wilczak, Jacek; Cetojevic-Simin, Dragana; Saric, Ljubisa; Teslic, Nemanja.
Afiliação
  • Maric B; University of Novi Sad, Institute of Food Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
  • Abramovic B; University of Novi Sad, Faculty of Science, Department of Chemistry, Biochemistry and Environmental Protection, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia.
  • Ilic N; University of Novi Sad, Faculty of Science, Department of Chemistry, Biochemistry and Environmental Protection, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia.
  • Bodroza-Solarov M; University of Novi Sad, Institute of Food Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
  • Pavlic B; University of Novi Sad, Institute of Food Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
  • Oczkowski M; University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
  • Wilczak J; Warsaw University of Life Sciences (WULS-SGGW), Institute of Human Nutrition Sciences, Department of Dietetics, Nowoursynowska 159c, 02-776 Warsaw, Poland.
  • Cetojevic-Simin D; Warsaw University of Life Sciences (WULS-SGGW), Institute of Veterinary Medicine, Department of Physiological Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.
  • Saric L; Singidunum University, Department of Pharmacy, Danijelova 32, 11000 Belgrade, Serbia.
  • Teslic N; University of Novi Sad, Institute of Food Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
Foods ; 12(1)2022 Dec 28.
Article em En | MEDLINE | ID: mdl-36613375
ABSTRACT
The primary aim of this experiment was to investigate the bioactivity potential and polyphenolic profile of defatted raspberry seeds (DRS) extracts from three varieties (Willamette, Meeker, and Polka) using the in vitro tests HPLC-DAD and UHPLC-Triple-TOF-MS. Extracts were obtained using ultrasound-assisted extraction (UAE) or hydrolysis. The antioxidant activity of the extracts was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic) cation (ABTS), and ferric reducing antioxidant power (FRAP) assays. Furthermore, the extracts were tested for antimicrobial activity using the disk diffusion method for four bacterial cultures (Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Salmonella enterica subsp. enterica Enteritidis). In vitro antiproliferative activity was tested using cervical carcinoma (HeLa), breast adenocarcinoma (MCF7), and fetal lung (MRC-5) human cell lines. In total, 32 phenolic compounds were detected in DRS extracts. A small quantity of ellagic acid (EA) was in free form, while EA content increased after the hydrolysis process. The extracts from the Meeker variety exhibited the highest antioxidant activity, analyzed with DPPH and FRAP assays, while extracts from the Polka variety had the highest activity towards ABTS•+ radical scavenging activity. The UAE samples expressed higher antiproliferative activity in comparison to hydrolysis extracts. The results indicate that DRS extracts have certain bioactivity, and their use in the food, cosmetic, and pharmaceutical industries is recommended.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article