Construction of a novel nano-enzyme for ultrasensitive glucose detection with surface-enhanced Raman scattering.
Spectrochim Acta A Mol Biomol Spectrosc
; 291: 122307, 2023 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-36630808
ABSTRACT
Fabricating more sensitive, stable and low-cost nanomaterials for the detection of glucose is important for the disease diagnosis and monitoring. Herein, we established a nanocomposite (polypyrrole bridging GO@Au@MnO2) as a novel surface-enhanced Raman scattering (SERS) nanoprobe for the quantitative detection of glucose in trace serum. Each component in the nanocomposites played an irreplaceable role in SERS detection of glucose. Polypyrrole (PPy) could act as Raman signal and extra SERS signal molecules didn't need to be introduced; Graphene oxide (GO) and gold nanoparticles (Au NPs) could enhance Raman signal of PPy; Au NPs also acted as glucose oxidase, which can oxidize glucose to produce gluconic acid and hydrogen peroxide(H2O2); Manganese oxide (MnO2) further enhanced Raman signal of PPy and responded to hydrogen peroxide, which will induce the decrease of Raman intensity of PPy. Thus, glucose can be quantified according to Raman signal output of PPy, which displayed a liner range from 1 to 10 µM, with detectable limit of 0.114 µM. Because of the merits in sensitivity, convenience and versatility, the novel method shows large potential space for disease-related substance detection in the future.
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Base de dados:
MEDLINE
Assunto principal:
Nanopartículas Metálicas
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article