Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation Sequencing.
Chem Res Toxicol
; 36(2): 162-176, 2023 02 20.
Article
em En
| MEDLINE
| ID: mdl-36647573
ABSTRACT
Recently, we constructed a hybrid thymine DNA glycosylase (hyTDG) by linking a 29-amino acid sequence from the human thymine DNA glycosylase with the catalytic domain of DNA mismatch glycosylase (MIG) from M. thermoautotrophicum, increasing the overall activity of the glycosylase. Previously, it was shown that a tyrosine to lysine (Y126K) mutation in the catalytic site of MIG could convert the glycosylase activity to a lyase activity. We made the corresponding mutation to our hyTDG to create a hyTDG-lyase (Y163K). Here, we report that the hybrid mutant has robust lyase activity, has activity over a broad temperature range, and is active under multiple buffer conditions. The hyTDG-lyase cleaves an abasic site similar to endonuclease III (Endo III). In the presence of ß-mercaptoethanol (ß-ME), the abasic site unsaturated aldehyde forms a ß-ME adduct. The hyTDG-lyase maintains its preference for cleaving opposite G, as with the hyTDG glycosylase, and the hyTDG-lyase and hyTDG glycosylase can function in tandem to cleave TG mismatches. The hyTDG-lyase described here should be a valuable tool in studies examining DNA damage and repair. Future studies will utilize these enzymes to quantify TG mispairs in cells, tissues, and genomic DNA using next-generation sequencing.
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MEDLINE
Assunto principal:
DNA Glicosilases
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Timina DNA Glicosilase
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Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article