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Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from Brassica napus L. Based on RNA-Seq and Metabolome.
Mi, Chao; Sun, Chao; Yuan, Yuting; Li, Fei; Wang, Qian; Zhu, Haiping; Hua, Shuijin; Lin, Liangbin.
Afiliação
  • Mi C; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China.
  • Sun C; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China.
  • Yuan Y; Agricultural Research Institute, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850032, China.
  • Li F; Yunnan Key Laboratory for Wild Plant Resources, Department of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
  • Wang Q; Horticultural Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650200, China.
  • Zhu H; Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650200, China.
  • Hua S; Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, 17, Hangzhou 310021, China.
  • Lin L; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China.
Plants (Basel) ; 12(2)2023 Jan 10.
Article em En | MEDLINE | ID: mdl-36679038
ABSTRACT
Brassica napus L. is a vital plant oil resource worldwide. The fatty acid biosynthesis and oil accumulation in its seeds are controlled by several genetic and environmental factors, including daytime and nighttime temperatures. We analyzed changes in oleic and erucic acid content in two double haploid (DH) lines, DH0729, a weakly temperature-sensitive line, and DH0815, a strongly temperature-sensitive line, derived from B. napus plants grown at different altitudes (1600, 1800, 2000, 2200, and 2400 m a.s.l., 28.85° N, 112.35° E) and nighttime temperatures (20/18, 20/16, 20/13 and 20/10 °C, daytime/nighttime temperature). Based on medium- and long-chain fatty acid metabolites, the total oleic acid content 35 and 43 days after flowering was significantly lower in low nighttime temperature (LNT, 20/13 °C) plants than in high nighttime temperature (HNT, 20/18 °C) plants (HNT 58-62%; LNT 49-54%; an average decrease of 9%), and the total erucic acid content was significantly lower in HNT than in LNT plants (HNT 1-2%; LNT 8-13%; an average increase of 10%). An RNA-seq analysis showed that the expression levels of SAD (LOC106366808), ECR (LOC106396280), KCS (LOC106419344), KAR (LOC106367337), HB1(LOC106430193), and DOF5 (LOC111211868) in STSL seeds increased under LNT conditions. In STSL seeds, a base mutation in the cis-acting element involved in low-temperature responsiveness (LTR), the HB1 and KCS promoter caused loss of sensitivity to low temperatures, whereas that of the KCS promoter caused increased sensitivity to low temperatures.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article