Dynamics and regulation of mitotic chromatin accessibility bookmarking at single-cell resolution.
Sci Adv
; 9(4): eadd2175, 2023 01 25.
Article
em En
| MEDLINE
| ID: mdl-36696508
ABSTRACT
Although mitotic chromosomes are highly compacted and transcriptionally inert, some active chromatin features are retained during mitosis to ensure the proper postmitotic reestablishment of maternal transcriptional programs, a phenomenon termed "mitotic bookmarking." However, the dynamics and regulation of mitotic bookmarking have not been systemically surveyed. Using single-cell transposase-accessible chromatin sequencing (scATAC-seq), we examined 6538 mitotic L02 human liver cells of variable stages and found that chromatin accessibility remained changing throughout cell division, with a constant decrease until metaphase and a gradual increase as chromosomes segregated. In particular, a subset of chromatin regions were identified to remain open throughout mitosis, and genes associated with these bookmarked regions are primarily linked to rapid reactivation upon mitotic exit. We also demonstrated that nuclear transcription factor Y subunit α (NF-YA) preferentially occupied bookmarked regions and contributed to transcriptional reactivation after mitosis. Our study uncovers the dynamic and regulatory blueprint of mitotic bookmarking.
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1
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Cromossomos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article