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Proteomic analysis of milk fat globule membranes from small-sized milk fat globules and their function in promoting lipid droplet fusion in bovine mammary epithelial cells.
Han, Liqiang; Huang, Qixue; Yang, JingNa; Lu, Wenyan; Hu, Mingyue; Yang, Yanbin; Zhu, Heshui; Pang, Kun; Yang, Guoyu.
Afiliação
  • Han L; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China. qlhan2001@126.com.
  • Huang Q; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China. qlhan2001@126.com.
  • Yang J; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China. qlhan2001@126.com.
  • Lu W; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China. qlhan2001@126.com.
  • Hu M; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China. qlhan2001@126.com.
  • Yang Y; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China. qlhan2001@126.com.
  • Zhu H; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China. qlhan2001@126.com.
  • Pang K; College of Animal Science and Veterinary Medicine, Xinyang Agriculture and Forestry University, Xinyang 464399, P. R. China.
  • Yang G; College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, P. R. China.
Food Funct ; 14(5): 2304-2312, 2023 Mar 06.
Article em En | MEDLINE | ID: mdl-36752527
ABSTRACT
In mammary epithelial cells, milk fat is synthesized as lipid droplets and secreted in the form of globules. Milk fat globules (MFGs) are covered by a lipid-protein membrane known as the milk fat globule membrane (MFGM). We randomly divided 12 Holstein cows into control and conjugated linoleic acid (CLA) groups. The control group was fed a basal diet, while the CLA group was fed the basal diet + CLA (15 g per kg DM) for 10 days. Cow performance, milk composition, and MFG size were measured daily. On day 10, we extracted MFGM proteins (n = 3) and identified them via quantitative proteomic analysis. We investigated the effects of the MFGM proteins from control and CLA-treated milk on the lipid droplet formation in MAC-T cells. Compared with the control group, the CLA group had reduced milk fat content (3.39 g/100 mL vs. 2.45 g/100 mL) and MFG size parameters (D[4,3] of 3.85 µm vs. 3.37 µm; D[3,2] of 3.24 µm vs. 2.83 µm). The specific surface area (SSA) increased in the CLA group. A total of 361 differentially expressed proteins were identified in the CLA group by iTRAQ quantitative proteomic analysis. Among these proteins, 100 were upregulated and 251 were downregulated (p < 0.05). In MAC-T cells, CLA-MFGM proteins increased the diameter of the lipid droplets to 1.32 µm. CLA-MFGM proteins decreased the proportion of the small lipid droplets (15.33% vs. 47.78%) and increased the proportion of the large lipid droplets (25.04% vs. 11.65%). CLA-MFGM proteins promoted lipid droplet fusion. Therefore, MFGM proteins play an important role in the regulation of the lipid droplet size.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Linoleicos Conjugados / Gotículas Lipídicas Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Linoleicos Conjugados / Gotículas Lipídicas Idioma: En Ano de publicação: 2023 Tipo de documento: Article