Fabrication and validation of an LED array microscope for multimodal, quantitative imaging.
HardwareX
; 13: e00399, 2023 Mar.
Article
em En
| MEDLINE
| ID: mdl-36756350
ABSTRACT
The combination of multiple imaging modalities in a single microscopy system can enable new insights into biological processes. In this work, we describe the construction and rigorous characterization of a custom microscope with multimodal imaging in a single, cost-effective system. Our design utilizes advances in LED technology, robotics, and open-source software, along with existing optical components and precision optomechanical parts to offer a modular and versatile design. This microscope is operated using software written in Arduino and Python and has the ability to run multi-day automated imaging experiments when placed inside of a cell culture incubator. Additionally, we provide and demonstrate methods to validate images taken in brightfield and darkfield, along with validation and optimization for differential phase contrast (DPC) quantitative phase imaging.
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MEDLINE
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En
Ano de publicação:
2023
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Article