Establishment of a novel 70K Mac-2 binding protein antibody through screening of fucosylation-related antibodies.

Masuda, Mika; Asuka, Tatsuya; Terao, Naoko; Nishino, Shinsuke; Ikeda, Shun; Takamatsu, Shinji; Kondo, Jumpei; Miyoshi, Eiji

Masuda, Mika; Asuka, Tatsuya; Terao, Naoko; Nishino, Shinsuke; Ikeda, Shun; Takamatsu, Shinji; Kondo, Jumpei; Miyoshi, Eiji.

Afiliação

Masuda M; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.
Asuka T; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.
Terao N; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.
Nishino S; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.
Ikeda S; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.
Takamatsu S; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.
Kondo J; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.
Miyoshi E; Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita 565-0871, Osaka, Japan.

J Biochem ; 173(6): 487-495, 2023 May 29.

Article em En | MEDLINE | ID: mdl-36760066

Assuntos

Glicoproteínas; Glicoproteínas de Membrana; Humanos; Anticorpos/metabolismo; Glicosilação; Células HEK293; Glicoproteínas de Membrana/metabolismo

Palavras-chave

70K Mac-2bp. Abbreviations: AAL, Aleuria aurantia lectin; Mac-2bp, Mac-2 binding protein; Mac-2bp OE, Mac-2bp over expression; 19-8H mAb, 19-8H monoclonal antibody; Mac-2bp pAb, Mac-2bp polyclonal antibody; CA19-9, carbohydrate antigen 19-9; GMDS, GDP-mannose 4,6-dehydratase; ELISA, enzyme-linked immunosorbent assay; Mac-2bp OE cells, Mac-2bp over-expressing HEK293T; Mac-2bp OE and GMDS KO cells, Mac-2bp overexpressing HEK293T with GMDS knockout cells; Mac-2 binding protein; fucosylation; glycan antibody; pancreatic cancer

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Glicoproteínas de Membrana / Glicoproteínas Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Glicoproteínas de Membrana / Glicoproteínas Idioma: En Ano de publicação: 2023 Tipo de documento: Article