Introduction of Genetic Mutations Into Mice by Base Editor and Target-AID.
Methods Mol Biol
; 2637: 111-124, 2023.
Article
em En
| MEDLINE
| ID: mdl-36773142
ABSTRACT
Generating genetically modified animal models that precisely recapitulate disease characteristics forms an integral and indispensable tool to understanding disease pathophysiology. Recently, important advances in genome editing technologies have enabled us to efficiently create sophisticated animal models in short periods of time. Base editing is a modified CRISPR/Cas system that induces base substitution at targeted genomic regions. Here I describe a basic protocol to introduce disease-relevant pathogenic mutations into mice utilizing two representative base editing tools, Base Editor and Target-AID.
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Base de dados:
MEDLINE
Assunto principal:
Sistemas CRISPR-Cas
/
Edição de Genes
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article