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Nursery outbreak caused by enteroaggregative Escherichia coli serogroup O111:H21.
Tré-Hardy, Marie; De Rauw, Klara; Blairon, Laurent; Simon, Anne; Piérard, Denis.
Afiliação
  • Tré-Hardy M; Department of Laboratory Medicine, Iris Hospitals South, Brussels, Belgium.
  • De Rauw K; Faculty of Medicine, Université Libre de Bruxelles, Brussels, Belgium.
  • Blairon L; Department of Pharmacy, Namur Research Institute for Life Sciences, University of Namur, Namur, Belgium.
  • Simon A; Department of Microbiology, General Hospital AZ Sint-Lucas, Ghent, Belgium.
  • Piérard D; Department of Laboratory Medicine, Iris Hospitals South, Brussels, Belgium.
APMIS ; 131(5): 206-216, 2023 May.
Article em En | MEDLINE | ID: mdl-36799765
This study describes, for the first time, the occurrence of an epidemic of enteroaggregative Escherichia coli (EAEC) O111:H21 in a Belgian nursery and describes a practical approach concerning its management. Few data exist in the literature on this type of outbreak. Clinical and microbiological investigations were needed to find a link between the cases and identify the causative agent. The microbiological procedure followed was first based on conventional analyses: isolation using selective cultures, identification by MALDI-TOF MS, antibiogram, determination of the serogroup by agglutination, then whole genome sequencing. A total of 7/21 children were infected with this pathogen. Four cases could be confirmed by a molecular technique, wgMLST, as belonging to the same bacterial clone. The action plan put in place focused on symptomatic case eviction, strict general hygiene precaution as well as specific cleaning and disinfection measures. The epidemic did last only a few days. It appears important, in the context of an epidemic, that clinical laboratories standardize their practice by equipping themselves with molecular techniques such as a multiplex which does not focus only on the serotype O157:H7 and which make it possible to distinguish the different pathotypes of E. coli by targeting several virulence genes (stx, aggR…). However, cost/effectiveness studies are awaited to confirm the interest of a systematic search by molecular method for the pathogen involved in a suspected outbreak occurring in a nursery.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Infecções por Escherichia coli Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Infecções por Escherichia coli Idioma: En Ano de publicação: 2023 Tipo de documento: Article