A micro-carbon nanotube transistor for ultra-sensitive, label-free, and rapid detection of Staphylococcal enterotoxin C in food.
J Hazard Mater
; 449: 131033, 2023 05 05.
Article
em En
| MEDLINE
| ID: mdl-36812728
ABSTRACT
Staphylococcal enterotoxin C (SEC) is an enterotoxin produced by Staphylococcus aureus, which can cause intestinal diseases. Therefore, it is of great significance to develop a sensitive detection method for SEC to ensure food safety and prevent foodborne diseases in humans. A field-effect transistor (FET) based on high-purity carbon nanotubes (CNTs) was used as a transducer, and a nucleic acid aptamer with high affinity was used for recognition to capture the target. The results indicated that the biosensor achieved an ultra-low theoretical detection limit of 1.25 fg/mL in PBS, and its good specificity was verified by detecting target analogs. Three typical food homogenates were used as the solution to be measured to verify that the biosensor had a swift response time (within 5 min after sample addition). An additional study with a more significant basa fish sample response also showed excellent sensitivity (theoretical detection limit of 8.15 fg/mL) and a stable detection ratio. In summary, this CNT-FET biosensor enabled the label-free, ultra-sensitive, and fast detection of SEC in complex samples. The FET biosensors could be further used as a universal biosensor platform for the ultrasensitive detection of multiple biological toxic pollutants, thus considerably stopping the spread of harmful substances.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Nanotubos de Carbono
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article