Optogenetic inhibition of the caudal substantia nigra inflates behavioral responding to uncertain threat and safety.
bioRxiv
; 2023 Feb 18.
Article
em En
| MEDLINE
| ID: mdl-36824795
ABSTRACT
Defensive responding is adaptive when it approximates current threat, but maladaptive when it exceeds current threat. Here we asked if the substantia nigra, a region consistently implicated in reward, is necessary to show appropriate levels of defensive responding in Pavlovian fear discrimination. Rats received bilateral transduction of the caudal substantia nigra with halorhodopsin or a control fluorophore, and bilateral ferrule implants. Rats then behaviorally discriminated cues predicting unique foot shock probabilities (danger, p =1; uncertainty, p =0.25; and safety, p =0). Green-light illumination (532 nm) during cue presentation inflated defensive responding of halorhodopsin rats - measured by suppression of reward seeking - to uncertainty and safety beyond control levels. Green-light illumination outside of cue presentation had no impact on halorhodopsin or control rat responding. The results reveal caudal substantia nigra cue activity is necessary to inhibit defensive responding to non-threatening and uncertain threat cues.
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MEDLINE
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En
Ano de publicação:
2023
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Article