Optimized protocols for chromatin immunoprecipitation of exogenously expressed epitope-tagged proteins.
STAR Protoc
; 4(1): 102050, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36853721
ABSTRACT
Chromatin immunoprecipitation (ChIP) assay is widely used for investigating the interaction between DNA and DNA-binding proteins such as transcription factors, co-factors, or chromatin-associated proteins. However, a successful ChIP assay largely depends on the quality of a ChIP-grade primary antibody. In cases where specific antibodies are unavailable or with low binding affinity, here, we describe a tailored protocol to achieve robust and reproducible chromatin binding by expressing an exogenous epitope-tagged protein in cells, followed by ChIP assays using a tag-specific antibody. For complete details on the use and execution of this protocol, please refer to Fang et al. (2021)1 and Kidder et al. (2011).2.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article