Comparative transcriptome analysis of wild type and a pectate lyase mutant strain of Bacillus subtilis.
New Microbiol
; 46(1): 52-55, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36853818
ABSTRACT
Transcriptome analysis for the original Bacillus subtilis K1 strain and UV mutagenic strain UW07 with high yield of pectate lyase was implemented with RNA-seq. The function of genes was annotated and metabolic pathways were classified to look for different expression genes and classify these genes into related metabolic pathways to reveal the high-yield mechanism of pectate lyase in UW07. The results showed that 397 genes were up-regulated and 617 genes were down-regulated compared with the original strain. The up-regulated genes were mainly involved in ABC transporters, two-component system, biosynthesis of amino acids, and carbon metabolism.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeo-Liases
/
Bacillus subtilis
/
Perfilação da Expressão Gênica
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article